Environ Microbiol. 2004, 6(7) 726-732

Link to the published paper

Lotte Lambertsen, Claus Sternberg, and Søren Molin*. Molecular Microbial Ecology Group, DTU Systems Biology, Technical University of Denmark, DK-2800 Lyngby, Denmark.

Abstract: The mini-Tn7 transposon system is a convenient tool for site-specific tagging of bacteria in which the tagging DNA is inserted at a unique and neutral chromosomal site. We have expanded the panel of mini-Tn7 delivery plasmids expressing different fluorescent proteins (stable and unstable) from the Escherichia coli lac derived promoter, PA1/04/03, or from the growth-rate-dependent Escherichia coli promoter PrrnB P1. The mini-Tn7 transposons were inserted and tested in the soil bacterium, Pseudomonas putida KT2440. Successful and site-specific tagging was verified by Southern blots as well as by PCR. Furthermore, the effect of fluorescent protein expression on the cellular growth rate was tested by growth competition assays.

• General Information about the Tn7 constructions
• How to use the Tn7 constructions
• Detailed description of the Tn7 plasmids

Please notice that we are unable to fullfill requests for the strains and plasmids. However, you are welcome to aquire the materials from any third party that may have them in their possession, without prior permission from us. If needed, please refer to this web-page.

For further information, please contact Søren Molin