Curr Protoc Microbiol, Chapter 1: p. Unit 1B 2.

Link to the published paper

Tolker-Nielsen1, T. and C. Sternberg* (2011). DTU Bioengineering, Technical University of Denmark, DK-2800 Lyngby, Denmark. *cst@bio.dtu.dk 1Current address: Copenhagen University.

We offer flowcells and bubbletraps on a non-profit basis. If you wish to buy flowcells or bubble traps, send an e-mail to Claus Sternberg including number of items required, shipping and invoicing information and VAT number. Please use the form provided below, or we may not be able to process your request. The original flowcell (figure 1A) is a piece of polycarbonate in which the channels are milled on a CNC tooling machine. The flowcell is designed in such a way that it will fit on a microscope stage. The flowcell is connected to the flow system using silicone tubing. The current version (Figure 1B) is a molded version made on a high-performance press.

Caution: The flow chambers and bubble traps can be autoclaved several times. However, when autoclaving the flow chambers they can bend slightly. To prevent this first place the flow chambers between two sheets of glass and autoclave for the first time. In our experience that prevents excessive bending in subsequent autoclave cycles.

The bubble trap (figure 2) is constructed from a single piece of polycarbonate tooled in the CNC machine. Using rubber rings, 5 mL syringes (Product no. BS-05S from Terumo® medical corp.) are attached onto each column. Bubbles are trapped in the syringe and prevented from disturbing the biofilm development.

Prices: Flowcell: 35 EUR Bubbletrap: 55 EUR (includes rubber rings, but not syringes or caps) Biofilm flow chambers and bubble traps are designed by DTU Bioengineering and manufactured on campus by a partner workshop.

Order form: Purchase Form (MS Word format)

Unfortunately, we are currently not able to sell the flow cells. We do not know if this will be possible at a later point.

If you use our flowcells, bubble traps, or designs you must credit DTU Bioengineering in your publication, e.g. in the methods and materials section. You can use the link to this web-page as reference, or the paper mentioned above.

We have prepared a small video which describes how to assemble the system. The video has been published by Journal of Visualized Experiments (JoVE) and is in the public domain (free). Link: Weiss Nielsen, M., C. Sternberg, S. Molin, and B. Regenberg, Pseudomonas aeruginosa and Saccharomyces cerevisiae biofilm in flow cells. J Vis Exp, 2011(47).

Technical Inquiries: Claus Sternberg