====== Mini-Tn7 transposons for site-specific tagging of bacteria with fluorescent proteins (2004). ======
//Environ Microbiol.// 2004, 6(7) 726-732{{ :envmic2004.jpg?100|}}

[[http://onlinelibrary.wiley.com/doi/10.1111/j.1462-2920.2004.00605.x/abstract|Link to the published paper]] 


Lotte Lambertsen, Claus Sternberg, and [[mailto:sm@bio.dtu.dk|Søren Molin]]*.
Molecular Microbial Ecology Group, DTU Systems Biology, Technical University of Denmark, DK-2800 Lyngby, Denmark. 
 
Abstract:
The mini-Tn7 transposon system is a convenient tool for site-specific tagging of bacteria in which the tagging DNA is inserted at a unique and neutral chromosomal site. We have expanded the panel of mini-Tn7 delivery plasmids expressing different fluorescent proteins (stable and unstable) from the Escherichia coli lac derived promoter, PA1/04/03, or from the growth-rate-dependent //Escherichia coli// promoter P//rrnB// P1. The mini-Tn7 transposons were inserted and tested in the soil bacterium, //Pseudomonas putida// KT2440. Successful and site-specific tagging was verified by Southern blots as well as by PCR. Furthermore, the effect of fluorescent protein expression on the cellular growth rate was tested by growth competition assays.
 
  * {{:tn7geninfo.pdf|General Information about the Tn7 constructions}} 
  * {{:tn7howto.pdf|How to use the Tn7 constructions}}
  * {{:tn7plasmids.pdf|Detailed description of the Tn7 plasmids}} 

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For further information, please contact [[mailto:sm@bio.dtu.dk|Søren Molin]]