|What is Comstat2?
Comstat is a program that analyzes image stacks of biofilms recorded by confocal microscopes. It can extract a number of quantitative parameters from biomass to fractal dimensions. The program does not contain any statistical calculation methods.
Comstat was developed by Arne Heydorn and Prof. Bjarne Ersbøll in 2000  as a MATLAB© script and has for many years been one of the preferred tools for biofilm quantification. In 2008 Martin Vorregaard ported and expanded the software  so it would run independently from MATLAB© but instead to utilize the public domain software Java© and the Image processing environment ImageJ (http://imagej.nih.gov/ij/) with the add-on package LOCI Tools (http://loci.wisc.edu/software/bio-formats). Comstat v. 2.1 is the latest build of this ImageJ plugin and this version was compiled and released July 1st 2015.
How to get Comstat2
Please follow this link to go to the sign up page. What you will have to do is to read the conditions, then add your name to the mailing list (you can unsubscribe at any time), confirm your e-mail addres and then download the software.
How to correctly cite the use of Comstat2
When publishing work which includes analyses made by Comstat2 we request that you will use these three references:
Comstat2 remains the property of the Technical University of Denmark. You can use the software for free by obeying the the license terms below:
This software is provided "as is" and any expressed or implied warranties, including, but not limited to, the implied warranties of merchantability and fitness for a particular purpose are disclaimed. In no event shall the regents or contributors be liable for any direct, indirect, incidental, special, exemplary, or consequential damages (including, but not limited to, procurement of substitute goods or services; loss of use, data, or profits; or business interruption) however caused and on any theory of liability, whether in contract, strict liability, or tort (including negligence or otherwise) arising in any way out of the use of this software, even if advised of the possibility of such damage.
You can contact us at firstname.lastname@example.org
If you prefer COMSTAT1 which runs as a MATLAB© script, please go to www.imageanalysis.dk
MATLAB© is a trademark of The Matworks Inc., Natick, MA